Jessay

 

 

자생 엉겅퀴 잎 추출물의

항산화효과 및 생리활성

Antioxidative and Physiological Activities of

Cirsium Japonicum var. ussuriense Leaf

Extract

 

 

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< 요약 및 결론 >

 

 

본 연구에서는

자생 엉겅퀴 잎의 생리활성 및 항산화 활성을 연구하고

이를 이용한 천연항산화제 개발을 위한

기초자료로 사용하기위하여

메탄올로 추출하였고,

자생 엉겅퀴 잎의 메탄올 추출물의

총 플라보노이드와 총 페놀 함량,

DPPH에 의한 전자공여능 측정,

아질산염 소거능,

SOD 유사활성능,

ferrous ion chelating 효과를 측정하였다.

 

1. 자생 엉겅퀴 잎의 메탄올 추출물의

총페놀의 함량과 총 플라보노이드의 함량은

각각 206.3 mg/g 및 303.4mg/g이었다.

 

 

2. 자생 엉겅퀴 잎의 메탄올 추출물의

아질산염 소거능 측정결과

pH 6.0에서 8.35%,

pH 3.0에서 28.29%,

pH 1.2에서 56.38%로,

pH가 낮아짐에 따라

아질산염 소거능이 높아지는 경향을 보였다. 

 

 

3. 자생 엉겅퀴 잎의 메탄올 추출물의

SOD 유사활성능은

0.5 mg/mL에서 19.27%,

1.0 mg/mL에서 21.35%,

2.0 mg/mL에에서 25.00%이었다.

농도가 증가함에 따라

SOD 유사활성능도 증가하는 경향을 보였지만

농도 증가폭에 비해

증가하는 SOD 유사활성능은 증가폭이 적었다.

 

 

4. 자생 엉겅퀴 잎의 메탄올 추출물의

ferrous ion chelaing 효과는

0.5 mg/mL에서 23.05%,

1 mg/mL에서 44.77%,

2 mg/mL에서 84.34%이었다.

결론적으로 자생 엉겅퀴 잎의

일반성분의 함량은 조회분 > 단백질 > 수분 > 지방의 순이었으며

각각 13.43%, 12.42%, 11.73% 및 7.87% 이었으며,

전자공여능, 아질산염 소거능, SOD 유사활성능 및 금속제거능은

시료의 농도가 높아질수록 그 효과가 높았다.

 

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